Effects of Tail Linkages on the Sequence-Specifi c Recognition of DNA by Hairpin Polyamide Conjugates
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Spectroscopic Investigation of Tetramethyl Rhodamine Quenching by N-methyl Imidazole, N-methyl Pyrrole, and b-alanine Linked Polyamides
The fluorescence from tetramethyl rhodamine in hairpin polyamide tetramethyl rhodamine (TMR) conjugates is quenched in solution. TMR fluoresces brightly upon polyamide binding match DNA. Similar behavior is not observed in the presence of mismatch DNA; the hairpin polyamide-fluorophore conjugates therefore provide a smallmolecule fluorescent means for detecting chosen DNA sequences in homogenou...
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A series of hairpin pyrrole-imidazole polyamide-fluorescein conjugates were synthesized and assayed for cellular localization. Thirteen cell lines, representing 11 human cancers, one human transformed kidney cell line, and one murine leukemia cell line, were treated with 5 microM polyamide-fluorescein conjugates for 10-14 h, then imaged by confocal laser scanning microscopy. A conjugate contain...
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Fluorescent methods for the sequence specific detection of DNA are vital to unraveling the functional, three-dimensional significance of the linear human genome. The ability of minor groove-binding polyamides to specifically target predetermined DNA sequences, without sample denaturation, in biologically relevant condensed states and protein complexes, offers appreciable advantages relative to ...
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Hairpin polyamide-chlorambucil conjugates containing an alpha-diaminobutyric acid (alpha-DABA) turn moiety are compared to their constitutional isomers containing the well-characterized gamma-DABA turn. Although the DNA-binding properties of unconjugated polyamides are similar, the alpha-DABA conjugates display increased alkylation specificity and decreased rate of reaction. Treatment of a huma...
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